Performance and Audio Interface – Bitwig Forum

In theory yes it could make a difference to performance, in practice it’s one of the last things I would focus on when trying to optimise performance. Thunderbolt interfaces can really only help reduce latency, and only a small amount due to avoiding the USB clock and instead reading directly from memory.

It depends on what the dsp performance graph in bitwig looks like to understand what might be the cause of the performance issues. If it’s spiking somewhat erratically even at high buffer sizes but low otherwise then likely other drivers on the PC are causing DPC latency, so you’d want to try updating drivers, or removing hardware and disabling drivers to test if it improves the situation and find the cause.